| 1. | Cloning of rejection related gene and construction of antisense gene transferring vector
玉米温敏核雄性不育相关基因克隆 |
| 2. | Construction of baculovirus transfer vectors for expression of core antigens of newcastle disease virus
核心抗原重组杆状病毒转移载体的构建 |
| 3. | Additionally , baculovirus has been a new gene transfer vector used in gene therapy due to its advantages of the extremely high expression of foreign genes and being non - reproduce vector
另外，杆状病毒是非复制型载体，能高效表达目的蛋白，其作为基因转移载体在基因治疗中乙显示出良好的应用前景。 |
| 4. | The transfer vector pbdtk - uni can be used for the construction of recombinant prv expressing foreign gene ( s ) . postgraduate : tianzhijun specialty : preventive veterinary science supervisors : prof . li yijing prof . tong guangzhi
以上结果表明所构建的具有自主知识产权的通用prv转移载体pbdtk - uni是成功的，为利用该载体构建伪狂犬病病毒二价基因工程疫苗提供了技术平台。 |
| 5. | The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak . 8 . bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
将克隆到的hbmp基因通过适当的酶切插入到转移载体质粒pbac - pak8的多克隆位点中，获得重组转移载体质粒pbacpak - hbmp 。 |
| 6. | The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol . to purify the recombinant virus , we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock
构建重组病毒：用已经构建好的angiostatin杆状病毒转移载体pbluebachiszb和病毒dna共同转染sfg细胞，通过蚀斑实验筛选出纯的重组斑点并扩增产生p二病毒贮存液。 |
| 7. | The chief aim of the present study was to primarily explore the early symbiotic interaction between m . huakuii and a . sinicus , using gfp and its mutants as reporter genes . the feasibility of broad - host - range plasmid ptr102 as a stable transfer vector in molecular biology of m huakuii was analyzed
采用发光酶发光活性和-葡萄糖苷酶组织染色法比较研究了携带报告基因luxab和gusa表达单元的ptr102标记质粒phn106和phn109在华癸中生根瘤菌7653r中的稳定性。 |
| 8. | Envelope gene gp85 of imc10200 subgroup j avian leukosis virus was cloned and expressed in the present study . the sequence encoding the gp85 domain of imc 10200 alv - j was amplified from pgem - imc2 . 2 vector , which contains env gene of alv - j imc 10200 strain , and cloned into transfer vector pfast bacl
为深入探讨alv - j的亚群特性，本研究利用alv - jgp85基因两侧的序列片段为引，物从正常spf蛋鸡、商品肉鸡和df1细胞基因组中完整地扩增了内源性类alv - jgp85基因。 |
| 9. | To obtain large amounts of appa phytase , the appa gene was subcloned into the prokaryotic expression vector pet - 28a ( + ) and baculovirus transfer vector pvl - 1393 under the control of the lac and polyhedrin promoter , respectively . the appa phytase was overexpressed in e . coli strain bl21 induced by lactose
为了大量表达appa植酸酶，我们将appa基因分别克隆至原核表达载体pet - 28a ( + )和杆状病毒转移载体pvl - 1393中，将其分别置于lac和polyhedrin启动子控制之下。 |
| 10. | In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection
此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。 |
